<div dir="ltr"><div dir="ltr"><div dir="ltr"><div class="gmail-textLayer" style="width:775px;height:1002px"><p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:11.5pt;font-family:Arial,sans-serif">The UW School of Medicine's Division
of Metabolism, Endocrinology & Nutrition in collaboration with EnTox
Sciences has an outstanding opportunity open for a Research Scientist in the
Sweet Laboratory.<span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:Arial,sans-serif">The overall goals of the Sweet/EnTox laboratory are to
facilitate and enhance sophisticated in vitro methodologies for research aimed
at understanding and treatment of diabetes and cancer. In particular, a fluidic
instrument that assesses real time responses of tissue function to test
compounds has been developed and is being beta-tested at collaborating
laboratories. Continued testing and optimization of the instrument based on feedback
are an important focus of the open position, and will ultimately lead to a
market-wide product launch.<span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:Arial,sans-serif">A broad range of techniques are needed to perform
experiments with the instruments including preparation of primary and cultured
tissue/cells, operation of the fluidics system, and supplemental assays from
samples generated by the instrument. Developed methodologies are subsequently
utilized in fundamental studies of intracellular mechanisms </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">mediating islet and retinal function. Studies relating
metabolism and bioenergetics, ion and membrane transport, mitochondrial
function, and cell signaling to secretion of insulin, glucose uptake in muscle
and survival of retina, are being carried out to validate the utility of the
methodologies and increase understanding of the biology of these systems.<span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:Arial,sans-serif">The position is particularly well suited to individuals
interested in transferring technology to the commercial sector and growing a
business, as well as those eager to perform basic research in intracellular
regulatory mechanisms of cell function. We are a small but very productive
laboratory/company that requires hard work. The effort is rewarded many-fold by
gaining multi-disciplinary expertise, participating in fast paced research and
development, publication of manuscripts, and daily accomplishments that support
high job satisfaction. <span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:11.5pt;font-family:Arial,sans-serif"> </span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:11.5pt;font-family:Arial,sans-serif">See the EnTox Sciences website,
<a href="https://www.entoxsciences.com/">https://www.entoxsciences.com/</a> for more information (although it is still under
construction) and <a href="https://pubmed.ncbi.nlm.nih.gov/?term=sweet+i&sort=date">https://pubmed.ncbi.nlm.nih.gov/?term=sweet+i&sort=date</a>
for past publications.<span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><b><span style="font-size:11.5pt;font-family:Arial,sans-serif">Major
responsibilities and Duties:</span></b><b><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
<br>
</span></b><b><span style="font-size:11.5pt;font-family:Arial,sans-serif"><span></span></span></b></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:11.5pt;font-family:Arial,sans-serif">Activities center on
performing/optimizing sophisticated real time methodologies to generate data to
support product development, scientific investigation, publications and grant applications.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
<br>
</span><span style="font-size:11.5pt;font-family:Arial,sans-serif"><span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:11.5pt;font-family:Arial,sans-serif">Specific Duties and
Responsibilities:</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Isolation of pancreatic islets,
retinal, muscle, hypothalamus and other tissue from rodents using microsurgical
and gradient purification techniques.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Culture of islets, tissue and cell
lines.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Assess metabolic and functional
viability of tissue/cells in flow culture using sophisticated, real-time spectroscopic
and imaging techniques.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Testing and characterization of a
fluidics systems that measures O </span><span style="font-size:7.5pt;font-family:"Courier New"">2 </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">consumption with a wide range of tissue including pancreatic
islets, retina, hypothalamus, muscle, liver and cultured cells.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Perform biochemical assays using
fluorometric, spectrophotometric and radiometric techniques.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Analyze and report experimental
results in laboratory meetings and in writing.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Order supplies as needed.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:"Courier New"">* </span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Compliance with regulation of drugs,
radioactivity and animal usage.<span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Minimum requirements: <span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:11.5pt;font-family:Arial,sans-serif">Experience in laboratory setting;
Bachelor's degree in a life science; willingness to work with rodents;
flexibility with work schedule; highly communicative and interactive.</span><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Pertinent expertise: Experience with cell culture,
harvesting of tissues from rodents and general life sciences laboratory
techniques; ability to operate analytical tools such as imaging microscopes,
spectrophotometers and plate readers; penchant and aptitude for engineering and
machine shop skills.<span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:12pt;font-family:"Times New Roman",serif"><br>
</span><span style="font-size:11.5pt;font-family:Arial,sans-serif">Salary commensurate with experience and education. Start
date is somewhat flexible; position is open until filled. <span></span></span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><span style="font-size:11.5pt;font-family:Arial,sans-serif"> </span></p>
<p class="MsoNormal" style="margin-bottom:0.0001pt;line-height:normal"><b><u><span style="font-size:11.5pt;font-family:Arial,sans-serif">If
interested, please send Resume and Brief Cover Letter to Ian Sweet,
<a href="mailto:isweet@u.washington.edu">isweet@u.washington.edu</a></span></u></b><b><u><span style="font-size:12pt;font-family:"Times New Roman",serif"><span></span></span></u></b></p></div></div></div></div>