[Biostudent] Employment - Upcoming/ Current graduates

[Biology Advising]

The UW School of Medicine's Division of Metabolism, Endocrinology &
Nutrition in collaboration with EnTox Sciences has an outstanding
opportunity open for a Research Scientist in the Sweet Laboratory.


The overall goals of the Sweet/EnTox laboratory are to facilitate and
enhance sophisticated in vitro methodologies for research aimed at
understanding and treatment of diabetes and cancer. In particular, a
fluidic instrument that assesses real time responses of tissue function to
test compounds has been developed and is being beta-tested at collaborating
laboratories. Continued testing and optimization of the instrument based on
feedback are an important focus of the open position, and will ultimately
lead to a market-wide product launch.


A broad range of techniques are needed to perform experiments with the
instruments including preparation of primary and cultured tissue/cells,
operation of the fluidics system, and supplemental assays from samples
generated by the instrument. Developed methodologies are subsequently
utilized in fundamental studies of intracellular mechanisms mediating islet
and retinal function. Studies relating metabolism and bioenergetics, ion
and membrane transport, mitochondrial function, and cell signaling to
secretion of insulin, glucose uptake in muscle and survival of retina, are
being carried out to validate the utility of the methodologies and increase
understanding of the biology of these systems.


The position is particularly well suited to individuals interested in
transferring technology to the commercial sector and growing a business, as
well as those eager to perform basic research in intracellular regulatory
mechanisms of cell function. We are a small but very productive
laboratory/company that requires hard work. The effort is rewarded
many-fold by gaining multi-disciplinary expertise, participating in fast
paced research and development, publication of manuscripts, and daily
accomplishments that support high job satisfaction.



See the EnTox Sciences website, https://www.entoxsciences.com/ for more
information (although it is still under construction) and
https://pubmed.ncbi.nlm.nih.gov/?term=sweet+i&sort=date for past
publications.


*Major responsibilities and Duties:*

Activities center on performing/optimizing sophisticated real time
methodologies to generate data to support product development, scientific
investigation, publications and grant applications.

Specific Duties and Responsibilities:
* Isolation of pancreatic islets, retinal, muscle, hypothalamus and other
tissue from rodents using microsurgical and gradient purification
techniques.
* Culture of islets, tissue and cell lines.
* Assess metabolic and functional viability of tissue/cells in flow culture
using sophisticated, real-time spectroscopic and imaging techniques.
* Testing and characterization of a fluidics systems that measures O 2
consumption
with a wide range of tissue including pancreatic islets, retina,
hypothalamus, muscle, liver and cultured cells.
* Perform biochemical assays using fluorometric, spectrophotometric and
radiometric techniques.
* Analyze and report experimental results in laboratory meetings and in
writing.
* Order supplies as needed.
* Compliance with regulation of drugs, radioactivity and animal usage.


Minimum requirements:

Experience in laboratory setting; Bachelor's degree in a life science;
willingness to work with rodents; flexibility with work schedule; highly
communicative and interactive.
Pertinent expertise: Experience with cell culture, harvesting of tissues
from rodents and general life sciences laboratory techniques; ability to
operate analytical tools such as imaging microscopes, spectrophotometers
and plate readers; penchant and aptitude for engineering and machine shop
skills.


Salary commensurate with experience and education. Start date is somewhat
flexible; position is open until filled.



*If interested, please send Resume and Brief Cover Letter to Ian Sweet,
isweet at u.washington.edu <isweet at u.washington.edu>*
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