[Biostudent] Student Employment -Sweet Laboratory.

[Biology Advising]

The UW School of Medicine's Division of Metabolism, Endocrinology &
Nutrition in collaboration with EnTox Sciences has an outstanding
opportunity open for a Research Scientist in the Sweet Laboratory.

The overall goals of the Sweet/EnTox laboratory are to facilitate and
enhance sophisticated in vitro methodologies for research aimed at
understanding and treatment of diabetes and cancer. In particular, a fluidic
instrument that assesses real time responses of tissue function to test
compounds has been developed and is being beta-tested at collaborating
laboratories. Continued testing and optimization of the instrument based on
feedback are an important focus of the open position, and will ultimately lead
to a market-wide product launch.  A broad range of techniques are
needed to perform
experiments with the instruments including preparation of primary and
cultured tissue/cells, operation of the fluidics system, and supplemental
assays from samples generated by the instrument.

Developed methodologies are subsequently utilized in fundamental
studies of intracellular
mechanisms mediating islet and retinal function. Studies relating metabolism
and bioenergetics, ion and membrane transport, mitochondrial function, and cell
signaling to secretion of insulin, glucose uptake in muscle and survival of
retina, are being carried out to validate the utility of the methodologies
and increase understanding of the biology of these systems.


The position is particularly well suited to individuals interested in
transferring technology to the commercial sector and growing a business, as
well as those eager to perform basic research in intracellular regulatory
mechanisms of cell function. We are a small but very productive laboratory/
company that requires hard work. The effort is rewarded many-fold by gaining
multi-disciplinary expertise, participating in fast paced research and d
evelopment, publication of manuscripts, and daily accomplishments that
support high job satisfaction. See the EnTox Sciences website,
https://www.entoxsciences.com/ for more information (although it is still
under construction) and
https://pubmed.ncbi.nlm.nih.gov/?term=sweet+i&sort=date for past publi
cations.

Major responsibilities and Duties:
Activities center on performing/optimizing sophisticated real time
methodologies to generate data to support product development, scientific
investigation, publications and grant applications.


Specific Duties and Responsibilities:
* Isolation of pancreatic islets, retinal, muscle, hypothalamus and
other tissue
from rodents using
microsurgical and gradient purification techniques.
* Culture of islets, tissue and cell lines.
* Assess metabolic and functional viability of tissue/cells in flow
culture using
sophisticated, real-time spectroscopic and imaging techniques.
* Testing and characterization of a fluidics systems that measures O 2
consumption with a wide range of tissue including pancreatic islets, retina,
hypothalamus, muscle, liver and cultured cells.
* Perform biochemical assays using fluorometric, spectrophotometric and r
adiometric techniques.
* Analyze and report experimental results in laboratory meetings and in
writing.
* Order supplies as needed.
* Compliance with regulation of drugs, radioactivity and animal usage.

Minimum requirements: Experience in laboratory setting; Bachelor's degree
in a life science; willingness to work with rodents; flexibility with work
schedule; highly communicative and interactive.

Pertinent expertise: Experience with cell culture, harvesting of tissues
from rodents and general life sciences laboratory techniques; ability to
operate analytical tools such as imaging microscopes, spectrophotometers and
plate readers; penchant and aptitude for engineering and machine shop skills
.


Salary commensurate with experience and education. Start date is somewhat
flexible; position is open until filled. If interested, please send Resume
and Brief Cover Letter to Ian Sweet, <isweet at u.washington.edu>
isweet at u.washington.edu

-- 
*Biology Advising - No in person meetings for WIN*
University of Washington, *BOX* 355320
318 Hitchcock Hall
http://www.biology.washington.edu/programs/undergraduate

*Zoom drop-in advising hours (10-15 min meetings):*

Monday - Thursday 9 a.m. - 12:00 p.m. and 1:00 p.m. - 4:00 p.m.

*Join Zoom Meeting *
*https://washington.zoom.us/j/92869479267
<https://washington.zoom.us/j/92869479267>*
Please include your name and student number in your emails.
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